Mitsa G, Guo Q, Goncalves C, Preston SEJ, Lacasse V, Aguilar-Mahecha A, Benlimame N, Basik M, Spatz A, Batist G, Miller WH Jr, Del Rincon SV, Zahedi RP, Borchers CH. J Biomol Tech. Explore targets and pathways in their scientific context, find and customize products to study them, analyze data and plan follow-up studies all in GeneGlobe. Here, we present a 'green' xylene-free protocol for accelerated sample preparation of FFPE tissues based on paraffin-removal with hot . h|Smk0+}2C%,+c[IN"K. Note: To determine if your sample contains endogenous peroxidase, read more about. Apply 100 l volume of primary and secondary antibodies. please visit our Contact Us page. Mansour A, Chatila R, Bejjani N, Dagher C, Faour WH. 88 0 obj <> endobj 103 0 obj <>/Filter/FlateDecode/ID[<10CDBFB44E95707131564288D4A135B0>]/Index[88 26]/Info 87 0 R/Length 81/Prev 171939/Root 89 0 R/Size 114/Type/XRef/W[1 2 1]>>stream Transfection Protocol . Comparison of this deparaffinization method with standard protocols, for example, xylene or Hemo-D with . Note: antigen retrieval conditions may require optimization. Going back to xylene will clear the slide and section. Aspirate fixative, rinse three times in 1X PBS for 5 min each. Mix briefly by vortexing, then add 10 l Proteinase K and mix by vortexing again. After deparaffinization, the core volume was approximately 0.4 mm, Representative tubes after deparaffinization. no. Evaluation of DNA extraction methods and real time PCR optimization on formalin-fixed paraffin-embedded tissues. For other support, Kit contents: Qiagen Deparaffinization Solution, 2 x 8mL, Non-odorous and is easily Tracked with its Blue Tracer Dye, For Deparaffinization of FFPE Samples. Cutting and mounting. Read more about. 2009 Dec 15;395(2):265-7. doi: 10.1016/j.ab.2009.08.016. Before "Deparaffinization of FFPE tissue in the Covaris E220 allows us to avoid the use of xylene in our small laboratory space. Would you like email updates of new search results? Prepare Proteinase K incubation mix. 24/7 automatic processing of online orders, Knowledgeable and professional Product & Technical Support. -, Ralton L.D., Murray G.I. . C. Deparaffinization Before proceeding with the staining protocol, the slides must be deparaffinized and rehydrated. Block each section with 100-400 l blocking solution for 1 hour at room temperature. 2014 Aug 8;1:90-5. doi: 10.1016/j.mex.2014.07.006. PMC Lin J, Kennedy SH, Svarovsky T, Rogers J, Kemnitz JW, Xu A, Zondervan KT. (, Efficient tissue homogenization using micropestles. Mol. Incomplete removal of paraffin can lead to poor staining of the section. Prepare a working solution of DAB and apply to tissue sections. Peptide samples were analyzed by nano-LC-MS/MS label-free quantitation (LFQ) to compare the performance of the evaluated protocols for each step of the sample preparation workflow. The present work aims to establish a deparaffinization and protein extraction protocol from FFPE kidney samples to obtain protein enough of high quality for the subsequent proteomic analysis. Pre-warm a jar with 10 mM Citric acid to 80C in water bath. Would you like email updates of new search results? Hematoxylin is used after deparaffinization and hydration. Hl[\ EkgQOP@A_hgmRu6`xDM+Rm]?wG}37\l&G/[2r[Vwc+T-^FxtVZSb4-.iq(%J^igSszS?suN9n8^N(vwz>ziVfm6^1LY7sXdbW[t./V ~wJ?%eW%d][=F~mb'v*ninm+E`>N6s5dT9d%x/;47lVjO.hWc3 deparaffinization and staining, and independent slide heating; Slide carousel holds 1-20 slides with independent temperature control for each position; Free standing or modified benchtop installation; 3. The TCGA protocol involves a combination of AllPrep DNA/RNA FFPE and High Pure (Roche) kits. ( A ) Efficacy of tryptic, Representative size of FFPE core used in this study. Proteom. If not specified, the recommended starting dilution is 2-5 g/ml. Description. It is uneccessary to pellet the FFPE sample after addition of Deparaffinization Solution or to remove paraffin-containing supernatant. Do NOT use with the Visium assay for snap frozen and OCT embedded tissue. This protocol outlines deparaffinization, Hematoxylin & Eosin (H&E) staining, imaging, and decrosslinking of tissue for use with 10x Genomics Visium Spatial Gene Expression for FFPE assay. Keywords: Remove antibody solution and wash sections in wash buffer three times . The basic steps of IHC (NBF/Par.) Unable to load your collection due to an error, Unable to load your delegates due to an error. -, Foll M.C., Fahrner M., Oria V.O., Kuhs M., Biniossek M.L., Werner M., Bronsert P., Schilling O. Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization. Download scientific diagram | Deparaffinization and rehydration protocol from publication: Measles virus infection enhances dendritic cell migration in a 3D environment | The respiratory system is . Optimize assays with customizable protocols and leverage automation to eliminate technician variability for reproducible, high quality stains. (For small rodent tissue, it is recommended to fix tissues for 4-8 hours.). 8600 Rockville Pike Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry. Deparaffinization Solution 20 ml: $24.20 -+ ADD TO CART Documents. 3 min. Tip: Before moving to alcohol grades step, make sure to completely deparaffinize the sections. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more. Summary of Findings: For mineral oil-deparaffinized specimens, decreasing the duration of proteinase K digestion from 72 h to 1 h had no effect on DNA yield, purity or mean DNA fragment size. The DAB reaction is permanent and stable and can be analyzed under a brightfield microscope at any time. 96 0 obj <>stream 8600 Rockville Pike It is uneccessary to pellet the FFPE sample after addition of . 89 0 obj <>/Filter/FlateDecode/ID[<893FC4B86E081446B755112D69A97264>]/Index[75 22]/Info 74 0 R/Length 77/Prev 246843/Root 76 0 R/Size 97/Type/XRef/W[1 3 1]>>stream PCR Amplifiable DNA from Breast Disease FFPE Section for Mutational Analysis. 2011 Oct 13;6(11):1695-709. doi: 10.1038/nprot.2011.388. Deparaffinized, stained, and decrosslinked tissue sections are inputs for the downstream Visium Spatial Gene Expression for FFPE workflow. This protocol outlines deparaffinization, decrosslinking, immunofluorescence (IF) staining, and imaging of tissue for use with 10x Genomics Visium Spatial Gene Expression for FFPE assay. If the antibody staining requires antigen retrieval to unmask the antigenic epitope refer below to section C. If antigen retrieval is not required proceed to section D. Make a working solution of Retrievagen A by mixing 18 ml of Retrievagen A solution 1 and 82 ml of Retrievagen A solution 2 and bring the final volume to 1 liter in distilled water. Materials and reagents Xylene 100% ethanol 95% ethanol Method Place the slides in a rack, and perform the following washes: 1. government site. The protocol described below is the Atlas Antibodies standard immunohistochemistry protocol optimized for Triple A Polyclonals and PrecisA Monoclonals. Effect of changing the deparaffinization protocol on DNA yield. Improved protocol for extraction of genomic DNA from formalin-fixed paraffin-embedded tissue samples without the use of xylene. Please enable it to take advantage of the complete set of features! Paraffin sections of 4 m thickness are baked overnight at 50C. doi: 10.1080/10520295.2018.1446101. 2018;93:373386. Place slides in a plastic coplin jar filled with the working Retrievagen solution and heat in a microwave oven to 203F (95C) (microwave oven ** or other heating sources such as pressure cooker (see alternate protocol), water bath can be used). To perform quantitative proteomics of FFPE samples, paraffin has to be removed and formalin-induced crosslinks have to be reversed prior to proteolytic digestion. QIAGEN Supplementary Protocol Sample & Assay Technologies Important points before starting Perform all centrifugation steps at room temperature (15-25C). Here, we present a 'green' xylene-free protocol for accelerated sample preparation of FFPE tissues based on paraffin-removal with hot water. Related research . (, An SDCTCEP-based buffer improves overall protein recovery from FFPE tissues. Clipboard, Search History, and several other advanced features are temporarily unavailable. Further . Before deparaffinization, place the slides in a 55C oven for ten minutes to melt the paraffin. . C.H.B. Deparaffinization. Deparaffinization and rehydration. Tip: The species of the animal serum used in permeabilization and blocking buffers is dependent on the host of your secondary antibody. endstream endobj startxref 50% Ethanol. Your browser does not have JavaScript enabled and some parts of this website will not work without it. IHC staining protocol for paraffin and frozen sectionsReagents can be applied manually by pipette, or this protocol can be adapted for automated and semi-automated systems if these are available.Carry out incubations in a humidified chamber to avoid tissue drying out, which will lead to non-specific binding and high background staining. Background The Fluorescence In Situ Hybridization (FISH) technique is a very useful tool for diagnostic and prognostic purposes in molecular pathology. IHC staining protocol Ventana Discovery XT. Add the primary antibody diluted in 1% animal serum in PBS (with or without 0.05-0.1 % Triton X 100) to the sections and incubate at room temperature for 1-2 hours. Additional Information. Proteomic Workflows for High-Quality Quantitative Proteome and Post-Translational Modification Analysis of Clinically Relevant Samples from Formalin-Fixed Paraffin-Embedded Archives. %%EOF Get resources and offers direct to your inbox. Experimental Design. 2. government site. 0 endstream endobj startxref 0 %%EOF 113 0 obj <>stream Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue. An official website of the United States government. This site needs JavaScript to work properly. Rinse with running tap water for 30-45 minutes. 1A. Question: How often should I refresh my deparaffinization and H&E staining solutions?. @bE@Hl79`" %X9(Fb? Geoffrey Rolls, BAppSc, FAIMS. JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. As soon as a brown color develops on the sections, immerse them in deionized water twice for 2 minutes each. Begin at step 5 and proceed through coverslipping. Deparaffinization of FFPE tissue blocks. (e.g. Deparaffinization of PFPE tissue sections with Deparaffinization Solution (PX12 June-15) page 3 of 5 5. This form is intended to help us improve our website experience. Then you will see white smears that are due to paraffinresidues. A Non-Hazardous Deparaffinization Protocol Enables Quantitative Proteomics of Core Needle Biopsy-Sized Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens. 2013;45:205218. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit (NEB #T2010) Materials and Equipment. official website and that any information you provide is encrypted Aspirate liquid, then cover cells to a depth of 2-3 mm with 4% formaldehyde diluted in warm PBS. sharing sensitive information, make sure youre on a federal *For an alternative Antigen Retrieval protocol using a vegetable steamer check protocol-ihc-paraffin-chromogenic. Dehydrate the tissue through 70%, 80%, 95% alcohol, 5 min each, followed with 3 times of 100% alcohol, 5 min each. Wash sections in wash buffer for 5 minutes. Immerse array slide in xylene for 10min, repeat once in new xylene for 10 min. Tech Tip: Deparaffinization and rehydration protocols can vary depending on the type/strength of reagents used as well as the intensity of the epitope retrieval procedure. Note: The SYSY standard protocol generates good staining results in the SYSY labs and may be used as suggestion . It stains the nucleus of the cell, specifically, the chromatin within the nucleus and the nuclear membrane. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. A widely used, standard deparaffinization protocol involving xylene was performed as a control. Counterstaining (If Desired) Dehydration and mounting. Note: If you are using an aqueous chromogen instead of DAB (i.e. Follow processing schedule recommended in section C, step 2. 10) Air dry slide and check slide for proper digestion; reveal dark distinguishable cells. Amino Acids. Deparaffinization with mineral oil: a simple procedure . Let tissues fix in . deparaffinization protocol This step is required when using paraffin embedded sections. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research, scientific journals, and education. 8) Place slide into Pepsin solution for 30 min. Tissue Sample, Paraffin. Wash the sections in distilled water two times for 5 minutes. ( A ) Total protein extracted after, Efficient tissue homogenization using micropestles., Efficient tissue homogenization using micropestles. Immunostaining tissue sections with fluorescently labeled antibodies enables simultaneous protein detection. MeSH Dressler FF, Schoenfeld J, Revyakina O, Vogele D, Kiefer S, Kirfel J, Gemoll T, Perner S. Clin Proteomics. The .gov means its official. 4. If the sections still have traces of wax, an additional immersion of 5 minutes in Xylene may be employed. -. Article Improved protocol for extraction of genomic DNA from formalin-fixed paraffin-embedded tissue samples without the use of xylene was published on December 1, 2016 in the journal Clinical Chemistry and Laboratory Medicine (CCLM) (volume 54, issue 12). Transfer slides to 100% alcohol, 2 changes for 3 minutes each and transfer once through 95% alcohol for 3 minutes. addition of lysis buffer to the tube containing the solvent, and centrifugation before lysis. Mix the working Retrievagen A solution in the coplin jar with a disposable pipet and incubate the slides at 203F for 10 minutes. 2. -, Maes E., Broeckx V., Mertens I., Sagaert X., Prenen H., Landuyt B., Schoofs L. Analysis of the formalin-fixed paraffin-embedded tissue proteome: Pitfalls, challenges, and future prospectives. You are now leaving the BD Biosciences website. Wash sections three times in PBS for 10 minutes each. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. The .gov means its official. IHC sample preparation (frozen vs. paraffin-embedded), IHC sample fixation (formalin vs. alcohol). The Addis et al. To View the Report, Please Follow These Steps: Extract all the contents of the Sample16SReport1.Zymo.zip . Unless otherwise noted, BD, the BD Logo and all other trademarks are the property of Becton, Dickinson and Company or its affiliates. If nuclear counterstaining is desired, use Hematoxylin according to the manufacturers instructions. Wash slides as indicated in step C5 above. Many protocols can be found in the literature and the websites of commercial antibody producers. Combine with AutoLys for a faster deparaffinization process. Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections. 2013 Apr;7(3-4):264-72. doi: 10.1002/prca.201200031. Place the slides in a rack, and perform the following washes using a Coplins jar: Once the slides have been washed in the above sequence, place slides in running cold tap water to rinse off ethanol. For antigen retrieval using a pressure cooker or an autoclave, pretreat slides with BD Retrievagen A solution in a glass or metal coplin jar as outlined in step C1 above. Immunohistochemistry is an important application of immunestaining in histology. This study aimed to deparaffinize formalin-fixed paraffin-embedded (FFPE) tissues using hot water instead of xylene and measuring the quantity and quality of the extracted DNA from the respective tissues. If these steps are not performed, the antibodies will not have complete access . Please enable it to take advantage of the complete set of features! ( A ) Total protein extracted from 1, An SDCTCEP-based buffer improves overall, An SDCTCEP-based buffer improves overall protein recovery from FFPE tissues. After 25 FFPE tissue samples were deparaffinized with the hot water method, DNA was then extracted. The, Representative tubes after deparaffinization. Bookshelf Deparaffinized, decrosslinked, and stained tissue sections are inputs for the downstream Visium Spatial Gene Expression for FFPE workflow. Purchase these through your usual distributor. Deparaffinization in EZ prep 75C 8 minutes. Accessibility eCollection 2014. Finally, our FFPE workflow does not require costly equipment and can be established in every standard clinical laboratory. Deparaffinization and Rehydration. Materials and ReagentsWaterbathContainer with iceGlass microscope slidesMicrotome and bladeOvenSectioningChill paraffin-embedded tissue blocks on ice before. An optimized xylene-free protein extraction method adapted to formalin-fixed paraffin embedded tissue sections for western blot analysis. Antigen retrieval/Pretreatment (If Necessary) Immunohistochemical staining. 1 0 obj<> endobj 3 0 obj<> endobj 4 0 obj<>/ProcSet[/PDF/Text]>>>> endobj 5 0 obj<> endobj 6 0 obj<> endobj 7 0 obj<> endobj 8 0 obj<> endobj 9 0 obj<> endobj 12 0 obj<> endobj 13 0 obj<> endobj 14 0 obj[/CalGray<>] endobj 15 0 obj[/CalRGB<>] endobj 16 0 obj null endobj 17 0 obj<> endobj 18 0 obj<> endobj 39 0 obj 555 endobj 40 0 obj<>stream 2 Immerse the slide into a staining dish containing xylene. HTn0?[D*)w}QmV+KJ'`[!4=1P\9d@Qr0;` s&83PsTUP>\;ghC DF-C^T {>c. deparaffinization and staining, and independent slide heating; Slide carousel holds 1-20 slides with independent temperature control for each position; Free standing or modified benchtop installation; Epub 2021 Jan 14. Kuras M, Woldmar N, Kim Y, Hefner M, Malm J, Moldvay J, Dme B, Fillinger J, Pizzatti L, Gil J, Marko-Varga G, Rezeli M. J Proteome Res. All Rights Reserved. Block endogenous peroxidase activity by incubating sections in 3% H2O2solution in methanol for 10 minutes. The Visium Spatial Gene Expression for FFPE is designed to measure mRNA in tissue sections derived from formalin fixed & paraffin embedded (FFPE) tissue samples and requires a Visium Spatial slide with intact tissue sections as input. 3. Importantly, this small volume is already compatible with tissue micro array (TMA) cores and core needle biopsies, while our results and its ease-of-use indicate that further downsizing is feasible. Agonists, activators, antagonists and inhibitors. Xenografts were generated, Experimental Design. 2. Incomplete removal of paraffin can lead to poor staining of the section. This protocol is only compatible with Spatial Gene Expression for FFPE reagent kits. A convenient tool to build experimental workflows and find products to match your needs. Optimize assays with customizable protocols and leverage automation to eliminate technician variability for reproducible, high quality stains. An official website of the United States government. Epub 2009 Aug 19. This study aimed to deparaffinize formalin-fixed paraffin-embedded (FFPE) tissues using hot water instead of xylene and measuring the quantity and quality of the extracted DNA from the respective tissues. Washing buffer between the steps is Reaction buffer. Biotech. Comprised of pretreated tubes and a rack system to reduce pipetting steps, the system eliminates the need for hazardous chemicals and minimizes loss of tissue sample. This emphasizes the necessity of a standardized FISH protocol with a high hybridization efficiency . Proceed to the next step when the intensity of the signal is appropriate for imaging. Tissues to be fixed and processed should be cut to a size no larger than 3 mm thick. To deparaffinize the tissue sections with hot water, small sections were exposed to 90 C disti Sample to Insight solutions for successful molecular analysis, Critical factors for molecular analysis of FFPE samples, This site is protected by reCAPTCHA and the Google. Try to go very quick through xylene into the 100% and 96% ethanol. Deparaffinization and hydration: For a sufficient reaction between antibody and antigen, deparaffinization steps should be: 1. After deparaffinization, the core, Representative tubes after deparaffinization. Immerse the tissue in paraffin for 3 times, 5 min each. Prior to immunostaining, deparaffinization and hydration are performed in xylene and graded . A novel xylene-free deparaffinization method for the extraction of proteins from human derived formalin-fixed paraffin embedded (FFPE) archival tissue blocks. 50% Ethanol, two washes 10 minutes each. Let the slides cool on the bench-top for 30 minutes. hb```c``*f`f``b@ !& 8p c f;t `] KX|'008b`f`aiX 2 " p(D@ Transfer slides to 100% alcohol, 2 changes for 3 minutes each and transfer once through 95% alcohol for 3 . u{}i|B{`L %IU5G ZNEzDEW Bookshelf If . Deparaffinize and rehydrate by immersing the slides through the following solutions/ wells: Draw a circle on the slide around the tissue with a hydrophobic barrier pen. After deparaffinization, specimens were treated with proteinase K for 72 h or 1 h. DNA was extracted from all specimen using the QIAamp FFPE kit. We extracted DNA from 12 recent and old archival FFPE bone marrow trephine biopsies by use of a simple protocol on the basis of deparaffinization with molecular biology-grade mineral oil followed by DNA extraction with the Qiagen FFPE kit. [2] . V?WTAj The https:// ensures that you are connecting to the Keep the slides in the tap water until ready to perform antigen retrieval. Paraffin is efficiently removed from FFPE samples . Label-free quantitation of FFPE cores from human ductal breast carcinoma in situ (DCIS) xenografts with a volume of only 0.79 mm3 showed a high correlation between replicates (r2 = 0.992) with a median %CV of 16.9%. Always wear gloves and work in a fume hood when working with DAB. Before Federal government websites often end in .gov or .mil. . This protocol outlines deparaffinization, decrosslinking, immunofluorescence (IF) staining, and imaging of tissue for use with 10x Genomics Visium Spatial Gene Expression for FFPE assay. 4. %PDF-1.5 % Block with Inhibitor CM, 37C 4 minutes. Rinse slides in PBS 3X, 5 minutes each time. Deparaffinization Solution provide a xylene-free method of removing paraffin from FFPE samples for DNA & RNA purification. B. Deparaffinization and re-hydration of tissue slide: Before deparaffinization, place the slides in a 55C oven for ten minutes to melt the paraffin. protocol are as follows: Fixation and paraffin embedding. 2021 Mar 24;10(1):1993. doi: 10.4081/jphr.2021.1993. Deparaffinization Solution, supplied by Qiagen, used in various techniques. Speed up your deparaffinization process with the Applied Biosystems AutoLys system. The parameters of the box plot are as in Fig. The use of formalin fixed wax embedded tissue for proteomic analysis. Rinse the tissue with running tap water for 5 min. In the procedure below, indicates the volumes to use if processing 1-2 sections per hb```"%YO>1FA 5c?t^_:xva`p H- - j8jaj"%!:a;@VF,FEl,L"2XZ mT06fRR`4)`TbFAA) 76-a%30 i^ wd,p35*t$q-0%SNYt@` B endstream endobj 89 0 obj <> endobj 90 0 obj <>/Rotate 0/Type/Page>> endobj 91 0 obj <>stream At no time from this point onwards should the slides be allowed to dry. Copyright 2016 Elsevier Inc. All rights reserved. Claire Josse and colleagues from the Human Genetic Laboratory at the GIGA - University of Lige have developed a new protocol combining the Bioruptor Pico with the AllPrep . . Note:The processing, embedding and sectioning of paraffin blocks requires specialized equipment and expertise and is usually performed by a histology or pathology laboratory. **Heating by use of microwave oven may require a license under US patent No. Disclaimer, National Library of Medicine US EN. 2017 Mar;32(3):307-313. doi: 10.14670/HH-11-789. Continue the incubation overnight at 4C in a humidified chamber. 9) Rinse slide in 70% ethanol 30 second. If the sections still have traces of wax, an additional immersion of 5 minutes in Xylene may be employed. Allow the slides to dry overnight and store slides at room temperature until ready for use. Most human tumor tissues that are obtained for pathology and diagnostic purposes are formalin-fixed and paraffin-embedded (FFPE). Incomplete removal of paraffin can lead to poor staining of the section. Remove blocking solution and add 100-400 l primary antibody diluted in recommended antibody diluent to each section. is the Chief Executive Officer of MRM Proteomics Inc. For routine diagnosis, the use of Hematoxylin and Eosin (H&E) is by far preferred for viewing cellular and tissue structure detail by pathologists. official website and that any information you provide is encrypted 2021;2261:525-533. doi: 10.1007/978-1-0716-1186-9_33. HHS Vulnerability Disclosure, Help Use the recommended dilution specified on the datasheet of the secondary antibody. A central component of almost all deparaffinization protocols is xylene, a toxic and highly flammable solvent that has been reported to negatively affect protein extraction and quantitative proteome analysis. Deparaffinization Solutionis optimized for deparaffinization prior to DNA or RNA purification from formalin-fixed paraffin-embedded tissue sections. 2019;1897:253-268. doi: 10.1007/978-1-4939-8935-5_22. Description. Deparaffinization and re-hydration of tissue slide 1. Use the BOND RX to confidently complete IHC, ISH, FISH, CTC, multiplexing and other tests.Explore your ideas in a variety of ways via open reagents, open detection kits, and customizable protocols.The BOND RX provides an easy way for labs to fully automate tests and accelerate research programs. Looks like you're visiting us from {{countryName}}. 2023 10x Genomics. J Proteomics. Speed up your next big discovery. Deparaffinization Solution. Immediately after deparaffinization, slides were rinsed in an aqueous wash composition containing PBS with 1% BRIJ-35 for 5 minutes, rinsed in tap water for 3 minutes, and used for immunohistochemistry. A widely used, standard deparaffinization protocol involving xylene was performed as a control.
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deparaffinization protocol